Biology: Insulin.

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BRIEF INTRODUCTIONInsulin is a protein hormone secreted from the Beta cells in the pancreas. Insulin is produced when there is a high level of sugar in the blood, insulin acts on cells in the body to help reduce blood sugar levels. Diabetics, who suffer from lack of insulin production, must rely on technology to produce insulin. Using Recombinant DNA Technology is one way artificial insulin can be produced. The processes for isolating and producing large amounts of insulin require specific enzymes, other bacteria and of course sophisticated technology. In this project, we 'll be discussing terms such as Restriction Enzymes they are specific proteins that speed up chemical reactions. Providing the right conditions they 'cut' DNA strands at specific locations, usually between the Guanine (G) and Adenine (A) bases. Restriction enzymes recognise specific sequences of DNA, and cut at these points. Similarly, another enzyme, called DNA Ligase, will also be discussed. The enzyme Ligase joins pieces of double stranded DNA at their sugar-phosphate backbones. The bonds that form here are strong (covalent) bonds. When isolating and cloning the gene - ligase, restriction enzymes and a vector, such as a plasmid found in bacteria, are used. A Vector (plasmid), is a small circular piece of DNA found in bacteria. Because bacteria multiply in large amounts by binary fission, they're used to manufacture and clone large amounts of insulin in a short amount of time, as the vector will have the insulin gene inserted. Thus cloning the insulin gene also. JOINING DNA FRAGMENTSIn gene manipulation, the joining of pieces of DNA with sticky ends to make one circular piece is sometimes required. The enzyme LIGASE (pic 71) joins pieces of double stranded DNA at their sugar-phosphate backbones. The bonds that form are strong (covalent) bonds.TRANSPORTING DNA INTO CELL

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Special carriers or vectors can transport DNA fragments into cells, such as bacteria. One example of a vector is a small circular DNA molecule, called a plasmid, found in bacteria. Under certain conditions plasmids can cross cell membranes and enter a cell. When it moves from outside to inside, it carries any passenger DNA which has been joined to it. Please note that this sample paper on Biology: Insulin. is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Biology: Insulin., we are here to assist you. Your

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The sexy fox

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Brush up on Windows skills and learn new ones. Whether you are a beginner or an advanced computer user, you are sure to find this section fascinating. It's packed with useful, easy-to-understand information and clear, step-by-step instructions. To get more out of Help and Support CenterIn many topics, you can click underlined words to see other information related to the topic.To see the definition of a green underlined term, click it. Try it here to see the definition of drag, and then click again to make the definition disappear.

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To see another topic, to open a program or dialog box, or to view a page on the Internet, click the blue underlined words. In Windows XP Home Edition, you must be logged on as a computer administrator to install components or modify Automatic Updates settings. In Windows XP Professional, you must be logged on as an administrator or a member of the Administrators group. If your computer is connected to a network, network policy settings might also prevent you from completing this procedure.Windows might prompt you to restart your computer after certain components are installed. Please restart your computer when prompted; otherwise, your computer might not work properly.Double-click the icon that appears in the notification area when you are notified that new updates are ready to be installed. Click Details. If you want an update to be installed, make sure that the check box beside it is selected. If you do not want an update to be installed, clear the check box beside it.Windows notifies you when installation is complete. NoteIf you choose not to install a specific update that has been downloaded, Windows deletes its files from your computer. If you change your mind later, you can restore it. To open System, click Start, click Control Panel, and then double-click System. On the Automatic Updates tab, click Restore Declined Updates. To view topics containing related information, click Related Topics, which often appears at the end of a topic, and then click the title of the topic you want. (If only one topic is related, you'll go directly there.) Troubleshooters are topics specially designed to help you diagnose and troubleshoot problems. Each page of a troubleshooter enables you to specify the symptoms you are experiencing, so that your problem can be narrowed down and fixed. Please note that this sample paper on The sexy fox is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on The sexy fox, we are here to assist you. Your persuasive essay on The sexy fox will be written from scratch, so you do not have to worry about its originality.

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What I did on my summer vacation

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During the summer between my Junior and Senior years in College I went on the best vacation of my life. That summer I traveled to Western Europe for two and a half months, with a total budget of $,500. What I thought would be a trip that was about broadening my horizons and learning about other cultures, turned out to be an experience of learning about the dynamics of people and relationships and gaining a better understanding of myself. Joining me on this adventure were two of my closest friends from my childhood and five additional young men who went to college with one of my childhood friends.As it turned out, the four guys that I didn't know had very different ideas about what they wanted to do with their trip than did I. We spent our first week in London, Amsterdam, Copenhagen, Munich, and Berlin. During the days in each city, we saw nearly every sight there was to see and visited every museum we could find. Each night was filled with the same activity, alcohol. After two days in London which included every museum and sight imaginable I had already found myself tired of sight seeing. I thought to myself, the sights are great, but I want to meet the people of London. Well, we moved on to Amsterdam where there were more museums to be seen and more sights to visit. Again, I found myself tired of it all and wanting to hang out in parks anI found that they had less money than I did and that they had brought sleeping bags so that they could camp, when necessary.

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The trip started in London, with six of seven guys taking the same flight. We spent two days in London touring the sights including Big Ben, Picadilly Circus, British Parliament and others. Ultimately, we found London to have terrible food and to be too expensive, so we moved on to Amsterdam. In Amsterdam we found Please note that this sample paper on What I did on my summer vacation is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on What I did on my summer vacation, we are here to assist you. Your persuasive essay on What I did on my summer vacation will be written from scratch, so you do not have to worry about its originality.

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Data

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Our staff of freelance writers includes over 120 experts proficient in Data, therefore you can rest assured that your assignment will be handled by only top rated specialists. Order your Data paper at affordable prices!What is data?

The Latin word datum means something that is known. The plural of datum is data, which could be translated as things that are known. In this book, however, as in most books dealing with computing, we treat the word data as a singular collective noun. Although the words data and information are commonly used as synonyms, information implies data that has had some organisation to facilitate understanding.

Information may be true or it may be false. It is largely the responsibility of humans to ensure that the information we have contains the truth.

Databases

A database is a large collection of things that are known. To belong to this collection the things do require a certain amount of organisation. So we should think of a database as a large collection or a store of information (true or false). An information system is a database along with the means to effectively manipulate the information it contains. The means to manipulate the information is known as a database management system, usually abbreviated to DBMS.

Stores of information, and the means to manage them, are as old as mankind. Through the millennia they have taken many forms, including chants and songs, drawings on stone, and hand written records on clay, skins and papyrus. The development of fast and powerful computers, coupled with a parallel growth in software, has brought about a dramatic growth in the capacity and efficiency of modem information systems. The information systems in this book focus on computerised storage and management.

Common examples of the application of information systems today include automated libraries, airline booking, video hire, student record keeping, software help, supermarket stock control, and countless others.

Computerised information systems offer the following advantages over manual systems of the past

• Information stored on CDs and magnetic discs and tapes occupies a relatively small amount of space.

• Information can be accessed very quickly.

• The likelihood of human error can be greatly reduced.

• A computer can deal with what were previously long and boring tasks performed by humans.

• information can be made available to a large number of people simultaneously, in the one building or around the world.

• Sophisticated electronic methods can be used to protect the information in a database.

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Japanese Karate

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Japanese KarateIntroductionThere are four major types of karate- Chinese, Japanese, Korean, and Okinawan. We will be focusing on the Japanese style of karate.

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General InformationKarate is one of the most popular Oriental forms of unarmed combat calledmartial arts in which a person kicks or strikes with the hands, elbows, feet, or knees. Altough some styles also teach throwing techniques, arm and wrist locks, and use ofweapons such as the staff(bo) or knuckle dusters(tekko).HistoryKarate entered Japan at the beginning of this century. It became a meansetpreserving and developing the samurai spirit when Funakoshi Gichin wrote a code oftwenty precepts for it. As early as 400's B.C., Buddhist monks in India used a form ofkarate to defend themselves against wild animals.The Art of KarateThis is a quote- The ultimate aim of the art of Karate lies not in victory or defeat,but in the perfection of the characters of its participants ~ Unknown.CostumeThe costume was named gi. It was a tradition jacket and pants, usually of whitecanvas, and a long cotton sash or belt. The ability of the person determines what beltthey have. Begginers have white but can learn more and practice and obtain a black beltwhen they are an expert. Most schools only go up to 8th degree black belt.TournamentsIn tournaments, the students fight but stop just short of hitting eachother and thepoints are awarded by the judges who decide how much that blow would have affectedhim.TrainingThere are three main ares for training calisthenics, kumite, and kata. Calisthenics is to build up acrobatic flexibility and speen riforously. Kumite is training for technical, specific karate blow with the knuckles, the ball on the outside of the foot,or the 'chopping' edge of the hand. Katas are acrobatic forms of fighting with animaginary opponent. They always train in bare feet.FactsA karate school is called dojo. The Japanese word, karate, means empty hand. Theblows in karate are meant to attack sensitive critical areas such as the throat, stomach,groin, and more. These moves could seriously injure or cripple someone. Cho, Henry S. The World Book Encyclopedia, World Book Inc., Volume K, UnitedStates, 1Cook, Harry Samarai, 1, New York, NY.Encarta Encyclopedia, Karate, Microsoft, 17.Mitchell, David. Official Karate. David and Charles, 186. Please note that this sample paper on Japanese Karate is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Japanese Karate, we are here to assist you. Your persuasive essay on Japanese Karate will be written from scratch, so you do not have to worry about its originality.

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