Elodea Lab

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Dara Ghasemizadeh

Period 1 AP Biology

/1/0Dissolved Oxygen and Aquatic Primary Productivity Lab

College papers on Elodea Lab

Introduction Oxygen is essential to the lives of many organisms. In the atmosphere there is quite an abundance of oxygen. While in the aquatic environment there is not as much. In the aquatic environment there only exists oxygen in it's free state, or dissolved oxygen (DO). Both chemical and physical factors can affect the amount of DO in water. Factors such as salinity, pH currents, and especially temperature can affect the DO. Biological processes photosynthesis and respiration can also affect the amount of DO in the water. The measurement of DO usually determines whether the biological activities requiring oxygen are occurring. In this lab we will be using the aquatic plant Elodea in samples containing different amounts of DO. We will be performing the light and dark method to measure the amount of DO. The change in DO concentration over time is a measure of net productivity. The use of bottles placed in light and dark environments is necessary in this lab. The difference over time for the light and dark bottles is a measure of gross productivity. We will need to perform the Wrinkler method in our expirement. This involves adding various chemicals such as alkaline iodide and manganous sulfate to our samples. This should determine the amount of DO in the water. Hypothesis I believe that the nutrient enriched samples will have greater productivity than those that are non- enriched. I also predict light intensity to increase the amount of productivity.Purpose and Objectives1. To understand the physical and biological factors that affect the solubility of dissolved gasses in aquatic ecosystems.. To define primary productivity.. To describe the relationship between dissolved oxygen and the processes of photosynthesis and respiration as they affect primary productivity in an ecosystem.4. To understand the affect of light and nutrients on photosynthesis.Materials & Equipment- 4 glass containers- buret- ringstand and clamps- pond water with Elodea- rubber bands- aluminum foil- manganous sulfate (MnSO4)- potassium hydroxide- potassium iodide (KOH- KI)- Sulfuric acid (H SO4)- Sodium thiosulfate solution- Starch- Nitrogen enrichment solution Procedure As directed on "Dissolved Oxygen and Aquatic Primary Productivity Lab" Except For 60mL BOD bottle

Add 1 drops MnSO4 1 drops KOH- KI Cap bottle and mix.Teacher adds drops of MH SO 4 Titrate 0 mL sample (Fill sample bottle to 4cm)

Add -4 drops of starch Titrate with Na- Thiosulfate until clear.ResultsGross productivityNet productivityRespiration Rate % Light Gross N-Enriched Net N-Enriched 100% 0.15 0.1 0.06 0.1 65% 0.15 0.18 0.06 0.0 5% 0.1 0.14 0.04 0.06 10% 0.1 0.1 0.01 0.01 % 0.06 0.07 -0.0 -0.0 Discussion1. Yes, most of the samples are light limited. This is mainly to show the effects of light depletion on photosynthesis and respiration of Elodea. We found that the darkened plants showed less production in terms of dissolved oxygen (DO). . Yes, likewise the samples were nutrient- limited. More than half of the samples were non- nutrient enriched. This is to test the effects on nutrient- limited Elodea and its photosynthesis/ respiration rate. By being nutrient- limited might have an affect on the plant's use or need of DO.. I can determine that productivity in terms of light intensity is that the productivity is greater as the light intensity increases. I can determine that productivity in terms of nutrients is that as nitrogen enrichment causes the productivity to increase. As described on my graph the nutrient non- enriched sample showed less productivity than that of the nutrient enriched one.Conclusion I found my hypothesis to be correct. The nutrient enriched samples had a higher productivity rate than those non- enriched. I found this surprising. Since nitrogen is said to decrease the amount oxygen productivity in terms of respiration. Which is what we were measuring. I mentioned that an increase in light productivity creates greater oxygen productivity which is right. I believe we had obscured data because we added the wrong amount of a certain solution. We ended up having poor data. For this reason our calculations ended up being incomplete so we were unable to determine the exact amounts of respiration or gross and net productivity rates Please note that this sample paper on Elodea Lab is for your review only. In order to eliminate any of the plagiarism issues, it is highly recommended that you do not use it for you own writing purposes. In case you experience difficulties with writing a well structured and accurately composed paper on Elodea Lab, we are here to assist you. Your persuasive essay on Elodea Lab will be written from scratch, so you do not have to worry about its originality.

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